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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16693 | 3' | -57.6 | NC_004156.1 | + | 129682 | 0.66 | 0.916574 |
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Target: 5'- aGACGGUGgccuuugcCGGUGGCau-GUGCAaCAg -3' miRNA: 3'- gCUGCCAC--------GCCACCGcuuCGCGUaGU- -5' |
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| 16693 | 3' | -57.6 | NC_004156.1 | + | 149291 | 0.66 | 0.910923 |
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Target: 5'- aCGA-GGagGCGGUGGCGuagaAGGUgGCGUCGg -3' miRNA: 3'- -GCUgCCa-CGCCACCGC----UUCG-CGUAGU- -5' |
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| 16693 | 3' | -57.6 | NC_004156.1 | + | 192924 | 0.66 | 0.910923 |
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Target: 5'- -aGCGGUagacUGGUGGCGGcAGCGC-UCAc -3' miRNA: 3'- gcUGCCAc---GCCACCGCU-UCGCGuAGU- -5' |
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| 16693 | 3' | -57.6 | NC_004156.1 | + | 75450 | 0.68 | 0.845982 |
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Target: 5'- uGAUGGUgGCGGUGGUGAcaauggcaacauuacAGUG-AUCAa -3' miRNA: 3'- gCUGCCA-CGCCACCGCU---------------UCGCgUAGU- -5' |
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| 16693 | 3' | -57.6 | NC_004156.1 | + | 174218 | 1.09 | 0.003266 |
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Target: 5'- aCGACGGUGCGGUGGCGAAGCGCAUCAa -3' miRNA: 3'- -GCUGCCACGCCACCGCUUCGCGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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