Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16693 | 5' | -57.1 | NC_004156.1 | + | 146740 | 0.66 | 0.918361 |
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Target: 5'- aCCGAUGCuacCUGGAcauugagcCCACCGGCaaGAu -3' miRNA: 3'- aGGCUACGc--GACCU--------GGUGGUUGcgCU- -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 111962 | 0.67 | 0.888199 |
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Target: 5'- aCCGAgUGuCGUUGGACagugugGCCAAUGUGGa -3' miRNA: 3'- aGGCU-AC-GCGACCUGg-----UGGUUGCGCU- -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 77200 | 0.68 | 0.860286 |
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Target: 5'- uUUCGAguuuaUGCGCaucaacGGACUGCCAACGUGu -3' miRNA: 3'- -AGGCU-----ACGCGa-----CCUGGUGGUUGCGCu -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 150728 | 0.69 | 0.834942 |
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Target: 5'- aCCGAUGUGCaGcacaaguguccgccGACCACC-ACGCGu -3' miRNA: 3'- aGGCUACGCGaC--------------CUGGUGGuUGCGCu -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 66167 | 0.69 | 0.829318 |
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Target: 5'- gCCGaAUGCGUUggauuugaaGGACUACUAugGUGAc -3' miRNA: 3'- aGGC-UACGCGA---------CCUGGUGGUugCGCU- -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 18149 | 0.69 | 0.79569 |
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Target: 5'- gUCCGGUGUGCacGGACCcugcaaaaagACgGAUGCGGc -3' miRNA: 3'- -AGGCUACGCGa-CCUGG----------UGgUUGCGCU- -5' |
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| 16693 | 5' | -57.1 | NC_004156.1 | + | 174252 | 1.09 | 0.003797 |
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Target: 5'- gUCCGAUGCGCUGGACCACCAACGCGAc -3' miRNA: 3'- -AGGCUACGCGACCUGGUGGUUGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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