Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16694 | 3' | -50.2 | NC_004156.1 | + | 81393 | 0.66 | 0.9984 |
Target: 5'- gCCGUGAUcugcUGCUGAGcaUCU-GCaUUGUCu -3' miRNA: 3'- gGGUACUA----ACGGCUC--AGAuCG-AACAG- -5' |
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16694 | 3' | -50.2 | NC_004156.1 | + | 22172 | 0.71 | 0.958087 |
Target: 5'- --gGUGAUUGCCaugauGGGcUCUAGUUUGUCa -3' miRNA: 3'- gggUACUAACGG-----CUC-AGAUCGAACAG- -5' |
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16694 | 3' | -50.2 | NC_004156.1 | + | 142185 | 0.72 | 0.950091 |
Target: 5'- aCCCAagGAguccaaGCCGAGUCUAGCUc--- -3' miRNA: 3'- -GGGUa-CUaa----CGGCUCAGAUCGAacag -5' |
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16694 | 3' | -50.2 | NC_004156.1 | + | 178385 | 1.12 | 0.009526 |
Target: 5'- gCCCAUGAUUGCCGAGUCUAGCUUGUCu -3' miRNA: 3'- -GGGUACUAACGGCUCAGAUCGAACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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