Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16697 | 3' | -53.1 | NC_004156.1 | + | 82238 | 0.66 | 0.987117 |
Target: 5'- gCCUGuuccacauccACCGGCAGAUUGuuGAUucgAUUACAg -3' miRNA: 3'- aGGAC----------UGGCCGUCUAAC--CUG---UGAUGU- -5' |
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16697 | 3' | -53.1 | NC_004156.1 | + | 109330 | 0.67 | 0.981761 |
Target: 5'- cUCUGACCGGUg---UGaGCACUACAc -3' miRNA: 3'- aGGACUGGCCGucuaACcUGUGAUGU- -5' |
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16697 | 3' | -53.1 | NC_004156.1 | + | 68855 | 0.7 | 0.905883 |
Target: 5'- uUCCauUGACC-GCAGAUUGGAUGuCUAUAc -3' miRNA: 3'- -AGG--ACUGGcCGUCUAACCUGU-GAUGU- -5' |
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16697 | 3' | -53.1 | NC_004156.1 | + | 225786 | 0.75 | 0.692963 |
Target: 5'- cCCUaGACCGaGCGGAUgaaaUGGACAUUACc -3' miRNA: 3'- aGGA-CUGGC-CGUCUA----ACCUGUGAUGu -5' |
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16697 | 3' | -53.1 | NC_004156.1 | + | 196399 | 1.1 | 0.006931 |
Target: 5'- aUCCUGACCGGCAGAUUGGACACUACAa -3' miRNA: 3'- -AGGACUGGCCGUCUAACCUGUGAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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