Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16698 | 5' | -50.5 | NC_004156.1 | + | 178300 | 0.66 | 0.999191 |
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Target: 5'- uGCUCUuugguaGC-CGGUUCaUAgugGCCGGUGGc -3' miRNA: 3'- -CGAGA------UGuGCUAGG-AUaa-CGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 139874 | 0.66 | 0.999191 |
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Target: 5'- aCUCUACcaguGCGGagUCgUAUUGuCCGGUGu -3' miRNA: 3'- cGAGAUG----UGCU--AGgAUAAC-GGCCACu -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 149007 | 0.66 | 0.999014 |
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Target: 5'- aCUUUGCgACG-UCUga-UGCCGGUGAc -3' miRNA: 3'- cGAGAUG-UGCuAGGauaACGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 86656 | 0.66 | 0.999014 |
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Target: 5'- aGCUCUcgGCGGcUCU--UGCUGGUGAg -3' miRNA: 3'- -CGAGAugUGCUaGGAuaACGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 42448 | 0.66 | 0.999014 |
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Target: 5'- -gUCaACAUG-UCCUAUcuaaUGUCGGUGAu -3' miRNA: 3'- cgAGaUGUGCuAGGAUA----ACGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 91918 | 0.66 | 0.998535 |
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Target: 5'- gGCUCgcuugGCACGAUugcgcugCCUcucgauaucgguGUUGUCGGUGu -3' miRNA: 3'- -CGAGa----UGUGCUA-------GGA------------UAACGGCCACu -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 94105 | 0.67 | 0.99661 |
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Target: 5'- --aCUACGCaAUUCUGUUGCUGGUu- -3' miRNA: 3'- cgaGAUGUGcUAGGAUAACGGCCAcu -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 180222 | 0.67 | 0.99661 |
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Target: 5'- gGCg-UACACGuagaCUUGUUGUCGGUGGc -3' miRNA: 3'- -CGagAUGUGCua--GGAUAACGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 18339 | 0.68 | 0.994633 |
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Target: 5'- uCUCUGCcucuACGAauauggCCUcggaggccAUUGCCGGUGGa -3' miRNA: 3'- cGAGAUG----UGCUa-----GGA--------UAACGGCCACU- -5' |
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| 16698 | 5' | -50.5 | NC_004156.1 | + | 205478 | 1.12 | 0.00965 |
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Target: 5'- cGCUCUACACGAUCCUAUUGCCGGUGAu -3' miRNA: 3'- -CGAGAUGUGCUAGGAUAACGGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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