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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16699 | 5' | -51.5 | NC_004156.1 | + | 127113 | 0.67 | 0.995008 |
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Target: 5'- aAUCGGAUUGGCCacgauaucgUGAUUGAucaacuugaaggcucUCGCAu -3' miRNA: 3'- -UGGCCUAGCUGGca-------ACUAACU---------------AGCGU- -5' |
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| 16699 | 5' | -51.5 | NC_004156.1 | + | 184245 | 0.67 | 0.993889 |
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Target: 5'- uGCUGGAcUCGGuuGUUGAUgucAUCGUAc -3' miRNA: 3'- -UGGCCU-AGCUggCAACUAac-UAGCGU- -5' |
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| 16699 | 5' | -51.5 | NC_004156.1 | + | 205681 | 0.67 | 0.99195 |
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Target: 5'- uGCCGGcgAUUGGCCGUguacggugucUGAUUGAUuaggUGCGu -3' miRNA: 3'- -UGGCC--UAGCUGGCA----------ACUAACUA----GCGU- -5' |
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| 16699 | 5' | -51.5 | NC_004156.1 | + | 207345 | 0.7 | 0.961905 |
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Target: 5'- -aUGGAuUCGGCUGc-GAUUGAUCGCAu -3' miRNA: 3'- ugGCCU-AGCUGGCaaCUAACUAGCGU- -5' |
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| 16699 | 5' | -51.5 | NC_004156.1 | + | 207126 | 1.09 | 0.012512 |
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Target: 5'- aACCGGAUCGACCGUUGAUUGAUCGCAa -3' miRNA: 3'- -UGGCCUAGCUGGCAACUAACUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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