Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 167 | 3' | -59.1 | AC_000007.1 | + | 25326 | 0.67 | 0.378747 |
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Target: 5'- aCGUCCGCGACUgcguuuacuuauUUCUGUGCuaCaCCu -3' miRNA: 3'- -GCAGGCGCUGA------------AAGGCGCGcgGaGGu -5' |
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| 167 | 3' | -59.1 | AC_000007.1 | + | 23991 | 0.67 | 0.352851 |
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Target: 5'- cCGUcgCCGCcGCcuccCCGCGCGCCcCCAa -3' miRNA: 3'- -GCA--GGCGcUGaaa-GGCGCGCGGaGGU- -5' |
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| 167 | 3' | -59.1 | AC_000007.1 | + | 16229 | 0.67 | 0.347824 |
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Target: 5'- gGUCgGCGGCgguggcgacgugCUaCGCGCCUCCGc -3' miRNA: 3'- gCAGgCGCUGaaa---------GGcGCGCGGAGGU- -5' |
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| 167 | 3' | -59.1 | AC_000007.1 | + | 16114 | 0.69 | 0.275818 |
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Target: 5'- gCGUggGCGugUagUUGCGCGCCUCCu -3' miRNA: 3'- -GCAggCGCugAaaGGCGCGCGGAGGu -5' |
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| 167 | 3' | -59.1 | AC_000007.1 | + | 15961 | 0.71 | 0.207262 |
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Target: 5'- gCGUgCGCGGCacuaCCGCGCGCC-CUg -3' miRNA: 3'- -GCAgGCGCUGaaa-GGCGCGCGGaGGu -5' |
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| 167 | 3' | -59.1 | AC_000007.1 | + | 10522 | 1.08 | 0.000323 |
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Target: 5'- gCGUCCGCGACUUUCCGCGCGCCUCCAc -3' miRNA: 3'- -GCAGGCGCUGAAAGGCGCGCGGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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