Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1670 | 5' | -61 | NC_001347.2 | + | 145634 | 0.67 | 0.772394 |
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Target: 5'- -gACUGCggguguuucCCGGAGGGUC-CGCgCGACa -3' miRNA: 3'- uaUGGCG---------GGCCUCUCAGcGCG-GCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 184508 | 0.66 | 0.781127 |
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Target: 5'- -cGCCGCgCCGuguGAGAGUUccaggGcCGCCGACa -3' miRNA: 3'- uaUGGCG-GGC---CUCUCAG-----C-GCGGCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 105070 | 0.66 | 0.789742 |
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Target: 5'- -gGCCGUCCGGuGAGgagGaCGgCGACGa -3' miRNA: 3'- uaUGGCGGGCCuCUCag-C-GCgGCUGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 156666 | 0.66 | 0.798232 |
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Target: 5'- -cGCCGCCacccauggCGGcGGGUCGUGgCGGCu -3' miRNA: 3'- uaUGGCGG--------GCCuCUCAGCGCgGCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 38650 | 0.66 | 0.801591 |
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Target: 5'- -cGCCuGCCCGGcGucugcagcgccaccGUCGUGCCGGCc -3' miRNA: 3'- uaUGG-CGGGCCuCu-------------CAGCGCGGCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 201404 | 0.66 | 0.80659 |
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Target: 5'- -gACCG-CUGGAGGccuccuaagacGUCGCGaCCGugGa -3' miRNA: 3'- uaUGGCgGGCCUCU-----------CAGCGC-GGCugC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 175495 | 0.66 | 0.822882 |
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Target: 5'- cGUACUGCCUGGuuAGUU-CGCCGuCGc -3' miRNA: 3'- -UAUGGCGGGCCucUCAGcGCGGCuGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 187614 | 0.66 | 0.822882 |
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Target: 5'- -----aCCUGGAGGGUUGCGCCGuaACa -3' miRNA: 3'- uauggcGGGCCUCUCAGCGCGGC--UGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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