Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1670 | 5' | -61 | NC_001347.2 | + | 82801 | 0.67 | 0.736438 |
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Target: 5'- -gACCGCCCGGAcgugcucaaaccGcGUCGUgaGCgCGGCGg -3' miRNA: 3'- uaUGGCGGGCCU------------CuCAGCG--CG-GCUGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 75343 | 0.72 | 0.465149 |
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Target: 5'- -gGCCGCCCuGAGAagaaucCGCGUCGACGu -3' miRNA: 3'- uaUGGCGGGcCUCUca----GCGCGGCUGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 40613 | 0.67 | 0.745566 |
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Target: 5'- -gGCCGCcggcgaugCCGGGGuGUUGCGgaGACGg -3' miRNA: 3'- uaUGGCG--------GGCCUCuCAGCGCggCUGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 39327 | 0.72 | 0.44786 |
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Target: 5'- uUGCCGCUacuCGGAGGGgCGC-CCGGCGg -3' miRNA: 3'- uAUGGCGG---GCCUCUCaGCGcGGCUGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 38650 | 0.66 | 0.801591 |
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Target: 5'- -cGCCuGCCCGGcGucugcagcgccaccGUCGUGCCGGCc -3' miRNA: 3'- uaUGG-CGGGCCuCu-------------CAGCGCGGCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 28248 | 0.67 | 0.727231 |
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Target: 5'- cGUACUGgCUGGAacGUCgGCGCCGACc -3' miRNA: 3'- -UAUGGCgGGCCUcuCAG-CGCGGCUGc -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 2026 | 0.69 | 0.632419 |
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Target: 5'- -gGCCGCCggCGGGGA--CGCGCCGuGCGc -3' miRNA: 3'- uaUGGCGG--GCCUCUcaGCGCGGC-UGC- -5' |
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| 1670 | 5' | -61 | NC_001347.2 | + | 1711 | 0.71 | 0.509807 |
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Target: 5'- -cGgCGCUCGGAcggGAGcUGCGCCGGCGg -3' miRNA: 3'- uaUgGCGGGCCU---CUCaGCGCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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