Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16701 | 3' | -49.1 | NC_004156.1 | + | 36491 | 0.68 | 0.99893 |
|
Target: 5'- cUCGCUCUgguuggGUGCAaagguguaaaACACCGACa---- -3' miRNA: 3'- -GGCGAGAa-----CAUGU----------UGUGGCUGagaag -5' |
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| 16701 | 3' | -49.1 | NC_004156.1 | + | 21665 | 0.75 | 0.896637 |
|
Target: 5'- aCGCUcCUUGUACAGCGCCaguguuGAaUCUUCg -3' miRNA: 3'- gGCGA-GAACAUGUUGUGG------CUgAGAAG- -5' |
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| 16701 | 3' | -49.1 | NC_004156.1 | + | 18274 | 0.67 | 0.999138 |
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Target: 5'- gCCGCUCUgacguugacuuucaaGUGCGACGCCaagaauggauACUUUUCg -3' miRNA: 3'- -GGCGAGAa--------------CAUGUUGUGGc---------UGAGAAG- -5' |
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| 16701 | 3' | -49.1 | NC_004156.1 | + | 15799 | 0.69 | 0.997362 |
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Target: 5'- uUGUUCUUGUACG-CACUGACcgcCUUUa -3' miRNA: 3'- gGCGAGAACAUGUuGUGGCUGa--GAAG- -5' |
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| 16701 | 3' | -49.1 | NC_004156.1 | + | 7776 | 0.68 | 0.99893 |
|
Target: 5'- cCCGCUCUUGU----CACCGAUgCUg- -3' miRNA: 3'- -GGCGAGAACAuguuGUGGCUGaGAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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