Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16701 | 3' | -49.1 | NC_004156.1 | + | 223293 | 0.69 | 0.996887 |
Target: 5'- uCgGCUgUUGUucguCAGaguCCGACUCUUCu -3' miRNA: 3'- -GgCGAgAACAu---GUUgu-GGCUGAGAAG- -5' |
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16701 | 3' | -49.1 | NC_004156.1 | + | 178419 | 0.69 | 0.997362 |
Target: 5'- -gGCUCUUGUGCcAgACCG-CUCagUUCa -3' miRNA: 3'- ggCGAGAACAUGuUgUGGCuGAG--AAG- -5' |
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16701 | 3' | -49.1 | NC_004156.1 | + | 36491 | 0.68 | 0.99893 |
Target: 5'- cUCGCUCUgguuggGUGCAaagguguaaaACACCGACa---- -3' miRNA: 3'- -GGCGAGAa-----CAUGU----------UGUGGCUGagaag -5' |
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16701 | 3' | -49.1 | NC_004156.1 | + | 44440 | 0.67 | 0.999121 |
Target: 5'- -aGCUC-UGUGCAcagcaccucaaGCACCGGCaUCUg- -3' miRNA: 3'- ggCGAGaACAUGU-----------UGUGGCUG-AGAag -5' |
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16701 | 3' | -49.1 | NC_004156.1 | + | 213694 | 1.14 | 0.011379 |
Target: 5'- gCCGCUCUUGUACAACACCGACUCUUCg -3' miRNA: 3'- -GGCGAGAACAUGUUGUGGCUGAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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