Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16715 | 3' | -54.7 | NC_004166.1 | + | 22471 | 0.66 | 0.672852 |
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Target: 5'- -gGAGACAGguAuuuggUUGGCCCGGCu -3' miRNA: 3'- cgUUUUGUCguUcga--GACCGGGCCGc -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 18328 | 0.67 | 0.582364 |
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Target: 5'- cGCAGAuGCGGCAA---UUGGUUCGGCGg -3' miRNA: 3'- -CGUUU-UGUCGUUcgaGACCGGGCCGC- -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 22295 | 0.68 | 0.559982 |
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Target: 5'- cCAAAACucAGUgaccuccuuguaAAGCUC-GGCCCGGUGc -3' miRNA: 3'- cGUUUUG--UCG------------UUCGAGaCCGGGCCGC- -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 24398 | 0.69 | 0.494556 |
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Target: 5'- aGCAAAGCcgAGCAgaaGGCc---GCCCGGCGg -3' miRNA: 3'- -CGUUUUG--UCGU---UCGagacCGGGCCGC- -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 37911 | 0.69 | 0.463156 |
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Target: 5'- cGCGAAACAcCGGGC---GGCCCGGUa -3' miRNA: 3'- -CGUUUUGUcGUUCGagaCCGGGCCGc -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 5805 | 0.71 | 0.384057 |
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Target: 5'- cGCAGAuaaaaucacaaGCGGCAcacuuaaAGCUCUGGaaaUCGGCa -3' miRNA: 3'- -CGUUU-----------UGUCGU-------UCGAGACCg--GGCCGc -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 12179 | 0.72 | 0.300331 |
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Target: 5'- -aAGAACAGCAAGCauggaaugaauUCaUGGCauCCGGCGg -3' miRNA: 3'- cgUUUUGUCGUUCG-----------AG-ACCG--GGCCGC- -5' |
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| 16715 | 3' | -54.7 | NC_004166.1 | + | 22171 | 1.11 | 0.000568 |
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Target: 5'- aGCAAAACAGCAAGCUCUGGCCCGGCGu -3' miRNA: 3'- -CGUUUUGUCGUUCGAGACCGGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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