Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16721 | 3' | -48.6 | NC_004167.1 | + | 15216 | 0.67 | 0.898451 |
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Target: 5'- aUGUGAAuCCAUAucCGGAAACAGUCGUc -3' miRNA: 3'- cACGUUU-GGUAUc-GCUUUUGUCGGCA- -5' |
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| 16721 | 3' | -48.6 | NC_004167.1 | + | 20743 | 0.67 | 0.874309 |
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Target: 5'- uUGagaAAGCCGUGGCGAAugcucAAUGGCUGa -3' miRNA: 3'- cACg--UUUGGUAUCGCUU-----UUGUCGGCa -5' |
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| 16721 | 3' | -48.6 | NC_004167.1 | + | 12801 | 0.68 | 0.838 |
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Target: 5'- -gGCAGGCCAggccGCGAuaccucAGCAGCCu- -3' miRNA: 3'- caCGUUUGGUau--CGCUu-----UUGUCGGca -5' |
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| 16721 | 3' | -48.6 | NC_004167.1 | + | 26516 | 0.71 | 0.671115 |
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Target: 5'- uUGC--GCCAUGGCGGccacuaauguaauAAAUAGCCGg -3' miRNA: 3'- cACGuuUGGUAUCGCU-------------UUUGUCGGCa -5' |
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| 16721 | 3' | -48.6 | NC_004167.1 | + | 9653 | 0.73 | 0.541234 |
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Target: 5'- gGUGCAGGCCAUuuccGGCGGAucAGCGGCa-- -3' miRNA: 3'- -CACGUUUGGUA----UCGCUU--UUGUCGgca -5' |
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| 16721 | 3' | -48.6 | NC_004167.1 | + | 10567 | 1.08 | 0.003237 |
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Target: 5'- cGUGCAAACCAUAGCGAAAACAGCCGUu -3' miRNA: 3'- -CACGUUUGGUAUCGCUUUUGUCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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