Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1673 | 5' | -56.6 | NC_001347.2 | + | 145096 | 0.66 | 0.946968 |
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Target: 5'- aUCGUUAUCCCCUCucucacaauGUGGaCAUGg--- -3' miRNA: 3'- gAGCAGUAGGGGAG---------CACC-GUGCagaa -5' |
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| 1673 | 5' | -56.6 | NC_001347.2 | + | 66632 | 0.67 | 0.911377 |
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Target: 5'- gUCGUUGUCCuccucguCCUCGUGGCGguuguuucucCGUCg- -3' miRNA: 3'- gAGCAGUAGG-------GGAGCACCGU----------GCAGaa -5' |
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| 1673 | 5' | -56.6 | NC_001347.2 | + | 128327 | 0.68 | 0.866078 |
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Target: 5'- gCUCGUCGUCaCCCgcgUGUGGC-CGcCg- -3' miRNA: 3'- -GAGCAGUAG-GGGa--GCACCGuGCaGaa -5' |
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| 1673 | 5' | -56.6 | NC_001347.2 | + | 225875 | 0.69 | 0.835392 |
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Target: 5'- -gCGUUAUCCguuCCUCGUaGGCugGUCc- -3' miRNA: 3'- gaGCAGUAGG---GGAGCA-CCGugCAGaa -5' |
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| 1673 | 5' | -56.6 | NC_001347.2 | + | 64514 | 0.7 | 0.78427 |
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Target: 5'- gUCGUCugucucauUCCCCcCGUGGCGCGa--- -3' miRNA: 3'- gAGCAGu-------AGGGGaGCACCGUGCagaa -5' |
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| 1673 | 5' | -56.6 | NC_001347.2 | + | 111526 | 1.05 | 0.007384 |
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Target: 5'- gCUCGUCAUCCCCUCGUGGCACGUCUUu -3' miRNA: 3'- -GAGCAGUAGGGGAGCACCGUGCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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