Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 168 | 3' | -59.6 | AC_000007.1 | + | 9802 | 0.66 | 0.450176 |
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Target: 5'- cGGUCuugagACGGCGGAUGGucgacagaagcaCCauguccuuGGGUCCGGc -3' miRNA: 3'- cCUAG-----UGCCGCCUGCC------------GG--------CCUAGGCC- -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 35227 | 0.66 | 0.440606 |
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Target: 5'- cGGUCACGcCGGcAUGGCCGuaGUCCGu -3' miRNA: 3'- cCUAGUGCcGCC-UGCCGGCc-UAGGCc -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 5156 | 0.66 | 0.412607 |
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Target: 5'- ---cUACGGCGGACGGCCucaaaCCGu -3' miRNA: 3'- ccuaGUGCCGCCUGCCGGccua-GGCc -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 10355 | 0.7 | 0.243783 |
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Target: 5'- aGGGgccaGCGuaGGGUGGCCGGGgcUCCGGg -3' miRNA: 3'- -CCUag--UGCcgCCUGCCGGCCU--AGGCC- -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 18631 | 0.7 | 0.237662 |
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Target: 5'- cGGGUUacaacaACGGCGGACGGCCcuggcagcacaGGuuucugCUGGg -3' miRNA: 3'- -CCUAG------UGCCGCCUGCCGG-----------CCua----GGCC- -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 8514 | 0.71 | 0.198352 |
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Target: 5'- cGGuaccgCGCGGCGGGCGGUgGGccgCgGGg -3' miRNA: 3'- -CCua---GUGCCGCCUGCCGgCCua-GgCC- -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 10636 | 0.8 | 0.047236 |
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Target: 5'- gGGuAUCAUGGCGGACGaccgggguucgaaccCCGGAUCCGGc -3' miRNA: 3'- -CC-UAGUGCCGCCUGCc--------------GGCCUAGGCC- -5' |
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| 168 | 3' | -59.6 | AC_000007.1 | + | 10729 | 1.12 | 0.000175 |
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Target: 5'- uGGAUCACGGCGGACGGCCGGAUCCGGg -3' miRNA: 3'- -CCUAGUGCCGCCUGCCGGCCUAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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