Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1682 | 3' | -53.9 | NC_001347.2 | + | 183442 | 0.7 | 0.882298 |
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Target: 5'- aGCAGGUUguaGGUGGUGAGgcgcggauagcgCGCCUcGGCc -3' miRNA: 3'- -CGUCCAA---UCGCCGCUCa-----------GUGGAuCUG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 198377 | 0.7 | 0.876673 |
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Target: 5'- cGCAGGUgggUGGCGcGCGGGUCGCg----- -3' miRNA: 3'- -CGUCCA---AUCGC-CGCUCAGUGgaucug -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 184658 | 0.71 | 0.862015 |
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Target: 5'- uGCAGG-UAGUGGCGcaGGUguCCgaugAGACg -3' miRNA: 3'- -CGUCCaAUCGCCGC--UCAguGGa---UCUG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 75968 | 0.73 | 0.73979 |
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Target: 5'- aCGGGcu-GCGGCGAGUCGCCcAG-Cg -3' miRNA: 3'- cGUCCaauCGCCGCUCAGUGGaUCuG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 151273 | 0.73 | 0.73979 |
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Target: 5'- gGCGGGUggaGGC-GCGGGUCAUCUacggGGACa -3' miRNA: 3'- -CGUCCAa--UCGcCGCUCAGUGGA----UCUG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 117897 | 0.74 | 0.710434 |
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Target: 5'- aCGGGgcGGCGGCGGGUC-CCgugauGGCa -3' miRNA: 3'- cGUCCaaUCGCCGCUCAGuGGau---CUG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 59458 | 0.75 | 0.629911 |
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Target: 5'- -gAGGUggauGCGGUGcgguGUCGCCUGGGCa -3' miRNA: 3'- cgUCCAau--CGCCGCu---CAGUGGAUCUG- -5' |
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| 1682 | 3' | -53.9 | NC_001347.2 | + | 105099 | 1.13 | 0.003779 |
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Target: 5'- cGCAGGUUAGCGGCGAGUCACCUAGACg -3' miRNA: 3'- -CGUCCAAUCGCCGCUCAGUGGAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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