Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16893 | 5' | -51.1 | NC_004305.1 | + | 9946 | 0.66 | 0.852406 |
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Target: 5'- cCUGUCaguGC-AUCGGCUCGUgAAGCc -3' miRNA: 3'- cGAUAGau-CGuUGGCCGGGUAgUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 31721 | 0.66 | 0.842444 |
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Target: 5'- aGCUAUCUGGUGGCCGuuuaauuGCgCGUCuuauGUa -3' miRNA: 3'- -CGAUAGAUCGUUGGC-------CGgGUAGuu--CG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 26961 | 0.67 | 0.814791 |
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Target: 5'- -aUAUCgAGCGgcugACUGGCUgaGUCAAGCg -3' miRNA: 3'- cgAUAGaUCGU----UGGCCGGg-UAGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 12372 | 0.67 | 0.773766 |
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Target: 5'- uGCUAUCau-CAGCUaauaaGGCCCAcggagUCGAGCu -3' miRNA: 3'- -CGAUAGaucGUUGG-----CCGGGU-----AGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 35451 | 0.67 | 0.773766 |
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Target: 5'- aGCU---UGGCGccccaGCUGGCUCaAUCAAGCa -3' miRNA: 3'- -CGAuagAUCGU-----UGGCCGGG-UAGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 15657 | 0.7 | 0.646611 |
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Target: 5'- gGCgcUUUAGCAGCgCGuGCCCGugccgccucaacaaUCAGGCu -3' miRNA: 3'- -CGauAGAUCGUUG-GC-CGGGU--------------AGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 21314 | 0.7 | 0.634998 |
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Target: 5'- --aAUCUAGCAGgCGGCCaCAggaguuugcggaguUCAAGUu -3' miRNA: 3'- cgaUAGAUCGUUgGCCGG-GU--------------AGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 23984 | 0.73 | 0.469317 |
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Target: 5'- -gUGUCUuGCAAgUcGCCCAUCAAGCc -3' miRNA: 3'- cgAUAGAuCGUUgGcCGGGUAGUUCG- -5' |
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| 16893 | 5' | -51.1 | NC_004305.1 | + | 7404 | 1.15 | 0.000649 |
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Target: 5'- gGCUAUCUAGCAACCGGCCCAUCAAGCa -3' miRNA: 3'- -CGAUAGAUCGUUGGCCGGGUAGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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