Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16895 | 3' | -43.9 | NC_004305.1 | + | 3173 | 0.67 | 0.996495 |
Target: 5'- ---gGCAAAAAAGGAccagACGCUGAUCGc -3' miRNA: 3'- cgcgUGUUUUUUUCU----UGCGGUUAGCa -5' |
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16895 | 3' | -43.9 | NC_004305.1 | + | 26837 | 0.68 | 0.992922 |
Target: 5'- aGCGCcaacugauACGuuuGAAGGugGCCGGUCa- -3' miRNA: 3'- -CGCG--------UGUuuuUUUCUugCGGUUAGca -5' |
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16895 | 3' | -43.9 | NC_004305.1 | + | 20944 | 0.7 | 0.971346 |
Target: 5'- --cCAUAAAGAAGGcAGCGCCAuUCGUu -3' miRNA: 3'- cgcGUGUUUUUUUC-UUGCGGUuAGCA- -5' |
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16895 | 3' | -43.9 | NC_004305.1 | + | 33411 | 0.72 | 0.928288 |
Target: 5'- cGCGCACG-----AGAACGCgGAUCu- -3' miRNA: 3'- -CGCGUGUuuuuuUCUUGCGgUUAGca -5' |
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16895 | 3' | -43.9 | NC_004305.1 | + | 7112 | 0.77 | 0.742508 |
Target: 5'- aGgGCACAAAAAAAGAcccGCGCCAGc--- -3' miRNA: 3'- -CgCGUGUUUUUUUCU---UGCGGUUagca -5' |
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16895 | 3' | -43.9 | NC_004305.1 | + | 4190 | 1.11 | 0.009296 |
Target: 5'- uGCGCACAAAAAAAGAACGCCAAUCGUu -3' miRNA: 3'- -CGCGUGUUUUUUUCUUGCGGUUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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