Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
169 | 5' | -52.5 | AC_000007.1 | + | 10786 | 0.66 | 0.744588 |
Target: 5'- uGCGCuagcuuuuuuGGCCAcUGGCCGcgcgcggcguaagcgGuuAGGCUGGa -3' miRNA: 3'- -CGCG----------CCGGUcACCGGU---------------UuuUUCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 10367 | 0.66 | 0.694749 |
Target: 5'- aGgGUGGCCGG-GGCUccGGGGGCgAGg -3' miRNA: 3'- -CgCGCCGGUCaCCGGuuUUUUCGaUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 18066 | 0.68 | 0.613166 |
Target: 5'- gGCGCGaGCCGuGUcgcggGGCCAGAGAcGCa-- -3' miRNA: 3'- -CGCGC-CGGU-CA-----CCGGUUUUUuCGauc -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 13779 | 0.69 | 0.55519 |
Target: 5'- gGCGCgGGCgGGUGGUgCGAGu--GCUGGu -3' miRNA: 3'- -CGCG-CCGgUCACCG-GUUUuuuCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 8520 | 0.69 | 0.521155 |
Target: 5'- cGCGCGGCgGGcggugGGCCGcgGGGGUg-- -3' miRNA: 3'- -CGCGCCGgUCa----CCGGUuuUUUCGauc -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 5521 | 0.7 | 0.49782 |
Target: 5'- uGCGC-GCCAagGGCCAcgccgcgGAGGGGCUGGa -3' miRNA: 3'- -CGCGcCGGUcaCCGGU-------UUUUUCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 3852 | 0.7 | 0.47712 |
Target: 5'- cCGCGGgCGGUGGCUgcAGcGGCUGa -3' miRNA: 3'- cGCGCCgGUCACCGGuuUUuUCGAUc -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 5350 | 0.71 | 0.414769 |
Target: 5'- cGCGCuGGCCagGGUGcGCUu--GAGGCUGGu -3' miRNA: 3'- -CGCG-CCGG--UCAC-CGGuuuUUUCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 14194 | 0.73 | 0.339912 |
Target: 5'- aGCGCGgcGCCAGUGGCgGcggc-GCUGGg -3' miRNA: 3'- -CGCGC--CGGUCACCGgUuuuuuCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 16256 | 0.75 | 0.240203 |
Target: 5'- cGgGCGGCCAugcgGGCCGcucGAAGGCUGGc -3' miRNA: 3'- -CgCGCCGGUca--CCGGUu--UUUUCGAUC- -5' |
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169 | 5' | -52.5 | AC_000007.1 | + | 10851 | 1.11 | 0.000717 |
Target: 5'- cGCGCGGCCAGUGGCCAAAAAAGCUAGc -3' miRNA: 3'- -CGCGCCGGUCACCGGUUUUUUCGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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