miRNA: 3'- -GUC-GcUUGGGUGggUUCA-CGAAUGGA- -5'
miRNA: 3'- gUCGCUUGGGUGG-GUUCACGA--AUGGa -5'
miRNA: 3'- -GUCGCUUGGGUGGGUUCACGAAUGGA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16901 | 5' | -54 | NC_004305.1 | + | 35352 | 0.7 | 0.467612 |
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Target: 5'- aCAGaCaAGCCCAUuuGAGUaGCUUGCCUg -3' miRNA: 3'- -GUC-GcUUGGGUGggUUCA-CGAAUGGA- -5' |
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| 16901 | 5' | -54 | NC_004305.1 | + | 18361 | 0.76 | 0.180858 |
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Target: 5'- uGGUGGGCCUGCCgCGGGUGCUgcUGCCg -3' miRNA: 3'- gUCGCUUGGGUGG-GUUCACGA--AUGGa -5' |
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| 16901 | 5' | -54 | NC_004305.1 | + | 18327 | 1.08 | 0.000948 |
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Target: 5'- cCAGCGAACCCACCCAAGUGCUUACCUa -3' miRNA: 3'- -GUCGCUUGGGUGGGUUCACGAAUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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