Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16906 | 5' | -47 | NC_004305.1 | + | 28577 | 0.66 | 0.978963 |
Target: 5'- --cGGCCGACCGCU----UCCAcgcuuucuuauGCCAu -3' miRNA: 3'- gaaUUGGUUGGCGGuaauAGGU-----------UGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 40645 | 0.66 | 0.978963 |
Target: 5'- gCUUAACCAAUCGCuCAUgcgcUCCGcAUCu -3' miRNA: 3'- -GAAUUGGUUGGCG-GUAau--AGGU-UGGu -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 10500 | 0.66 | 0.969866 |
Target: 5'- --gAGCUAGCUGCUAacaugauUCCAGCCu -3' miRNA: 3'- gaaUUGGUUGGCGGUaau----AGGUUGGu -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 16778 | 0.67 | 0.962421 |
Target: 5'- gUUAACCAACCaGgCA--AUCCAAUCu -3' miRNA: 3'- gAAUUGGUUGG-CgGUaaUAGGUUGGu -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 33669 | 0.68 | 0.938476 |
Target: 5'- aCUUAAUCAucgUCGCCAUc-UCCAAUCAu -3' miRNA: 3'- -GAAUUGGUu--GGCGGUAauAGGUUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 5087 | 0.68 | 0.938476 |
Target: 5'- uUUGACUGGCCGUCA--GUuuGGCCAu -3' miRNA: 3'- gAAUUGGUUGGCGGUaaUAggUUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 23237 | 0.68 | 0.920249 |
Target: 5'- gCUUAGCaCGgaauGCuUGCCAUUGggugCCAGCCAa -3' miRNA: 3'- -GAAUUG-GU----UG-GCGGUAAUa---GGUUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 12813 | 0.69 | 0.899088 |
Target: 5'- ---uACCAACgUGcCCAgaGUCCAGCCAg -3' miRNA: 3'- gaauUGGUUG-GC-GGUaaUAGGUUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 5095 | 0.7 | 0.883389 |
Target: 5'- uUUGACC-ACCGCU---GUUCAGCCGu -3' miRNA: 3'- gAAUUGGuUGGCGGuaaUAGGUUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 12278 | 0.7 | 0.883389 |
Target: 5'- uUUAACCAGaguaCGCCGaucUUGUCCAAUa- -3' miRNA: 3'- gAAUUGGUUg---GCGGU---AAUAGGUUGgu -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 26387 | 0.71 | 0.838953 |
Target: 5'- gUUAACCAGUCGUUAUUGUCC-ACUAa -3' miRNA: 3'- gAAUUGGUUGGCGGUAAUAGGuUGGU- -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 24658 | 0.71 | 0.798721 |
Target: 5'- uCUUGACaguuuguuuGCCGCCAUUGUCUucACCu -3' miRNA: 3'- -GAAUUGgu-------UGGCGGUAAUAGGu-UGGu -5' |
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16906 | 5' | -47 | NC_004305.1 | + | 36866 | 1.1 | 0.004133 |
Target: 5'- gCUUAACCAACCGCCAUUAUCCAACCAg -3' miRNA: 3'- -GAAUUGGUUGGCGGUAAUAGGUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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