Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16911 | 3' | -55.3 | NC_004313.1 | + | 6817 | 0.66 | 0.638881 |
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Target: 5'- -gGCGGGGuaaaaggcaccguUGAuAUGCCgguugCCCuCGCCg -3' miRNA: 3'- cgCGCCCU-------------GCUuUAUGGa----GGGuGCGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 34152 | 0.66 | 0.628882 |
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Target: 5'- cGCGCuGGAUGGuucaaGCCgCCCgACGCUg -3' miRNA: 3'- -CGCGcCCUGCUuua--UGGaGGG-UGCGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 14941 | 0.67 | 0.606681 |
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Target: 5'- -aGCGGGAUGAcaggaC-CCCACaGCCa -3' miRNA: 3'- cgCGCCCUGCUuuaugGaGGGUG-CGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 3458 | 0.69 | 0.477667 |
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Target: 5'- uGCGUGGGGauaUGAAGUcCCguuuugaagCCUAUGCCa -3' miRNA: 3'- -CGCGCCCU---GCUUUAuGGa--------GGGUGCGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 10984 | 0.69 | 0.467477 |
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Target: 5'- cGCGCGGGAUGcguAUuCCUUCUGCaucgaGCCg -3' miRNA: 3'- -CGCGCCCUGCuu-UAuGGAGGGUG-----CGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 7315 | 0.71 | 0.390354 |
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Target: 5'- aUGCGGGugG-GAUGCUUCUCGaucugaacCGCCa -3' miRNA: 3'- cGCGCCCugCuUUAUGGAGGGU--------GCGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 11002 | 0.71 | 0.346566 |
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Target: 5'- aCGCuGGGCGA--UACCUUCCugccCGCCa -3' miRNA: 3'- cGCGcCCUGCUuuAUGGAGGGu---GCGG- -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 962 | 0.72 | 0.330063 |
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Target: 5'- gGCGUGGGAgGuauuuCgUCCCGCGCg -3' miRNA: 3'- -CGCGCCCUgCuuuauGgAGGGUGCGg -5' |
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| 16911 | 3' | -55.3 | NC_004313.1 | + | 1027 | 1.14 | 0.000352 |
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Target: 5'- cGCGCGGGACGAAAUACCUCCCACGCCu -3' miRNA: 3'- -CGCGCCCUGCUUUAUGGAGGGUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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