Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16911 | 5' | -55.2 | NC_004313.1 | + | 6163 | 0.66 | 0.637206 |
Target: 5'- uGUGGCGCUGG--------UCUGCGCCu -3' miRNA: 3'- -CACCGCGGCCucauaugaAGGCGUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 961 | 0.66 | 0.625946 |
Target: 5'- -aGGCGUgGGAG-GUAUUUcgucCCGCGCg -3' miRNA: 3'- caCCGCGgCCUCaUAUGAA----GGCGUGg -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 3348 | 0.66 | 0.614691 |
Target: 5'- gGUGGCGCgauCGGuGU-----UCUGCACCa -3' miRNA: 3'- -CACCGCG---GCCuCAuaugaAGGCGUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 37904 | 0.66 | 0.614691 |
Target: 5'- -aGGuCGCCGGAGUAU--UUCaGaCACCc -3' miRNA: 3'- caCC-GCGGCCUCAUAugAAGgC-GUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 10990 | 0.68 | 0.526005 |
Target: 5'- uGUGGcCGCgCGGGaugcGUAUucCUUCUGCAUCg -3' miRNA: 3'- -CACC-GCG-GCCU----CAUAu-GAAGGCGUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 9905 | 0.68 | 0.515217 |
Target: 5'- cUGGaGCUGGAGUuggagGCUgUCCGUAUCa -3' miRNA: 3'- cACCgCGGCCUCAua---UGA-AGGCGUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 16645 | 0.69 | 0.452597 |
Target: 5'- --uGCGuuGGGGUAUACcgCUGcCACCa -3' miRNA: 3'- cacCGCggCCUCAUAUGaaGGC-GUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 923 | 0.69 | 0.442562 |
Target: 5'- -cGGUGCgGaAGUAUACUcCgGCGCCa -3' miRNA: 3'- caCCGCGgCcUCAUAUGAaGgCGUGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 26983 | 0.69 | 0.422877 |
Target: 5'- -cGGCGUCGGAGU--GCUguaCUGCAgCu -3' miRNA: 3'- caCCGCGGCCUCAuaUGAa--GGCGUgG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 27797 | 0.71 | 0.324666 |
Target: 5'- -cGGCGUCGGAGUGUAaauaCCGUagggguuguACCg -3' miRNA: 3'- caCCGCGGCCUCAUAUgaa-GGCG---------UGG- -5' |
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16911 | 5' | -55.2 | NC_004313.1 | + | 989 | 1.13 | 0.000365 |
Target: 5'- cGUGGCGCCGGAGUAUACUUCCGCACCg -3' miRNA: 3'- -CACCGCGGCCUCAUAUGAAGGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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