Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16913 | 3' | -57.8 | NC_004313.1 | + | 11217 | 0.66 | 0.488837 |
Target: 5'- aGUCGCCau-UGCGgCGC--UGGUGGCc -3' miRNA: 3'- -CGGCGGacuACGCgGCGcuACUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 36975 | 0.67 | 0.418438 |
Target: 5'- gGCCGCCaaacagcagccugUGAaugGuUGCCGCGc-GGUGGCg -3' miRNA: 3'- -CGGCGG-------------ACUa--C-GCGGCGCuaCUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 8079 | 0.67 | 0.400663 |
Target: 5'- aCCaGCggUGAUGaCGCUGCGgcGGUGGCu -3' miRNA: 3'- cGG-CGg-ACUAC-GCGGCGCuaCUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 15430 | 0.68 | 0.391504 |
Target: 5'- uGCUGCCUGAUaUG-UGUGAUGAggacUGGCu -3' miRNA: 3'- -CGGCGGACUAcGCgGCGCUACU----ACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 6572 | 0.68 | 0.372719 |
Target: 5'- -aUGCgUGAUGCGCgGCGAcuggucaUGAaacgUGGCc -3' miRNA: 3'- cgGCGgACUACGCGgCGCU-------ACU----ACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 5215 | 0.68 | 0.361401 |
Target: 5'- -gCGCCgguacGAUGCGCCuuacgcaGCGuguaaaucagcuuaAUGAUGGCa -3' miRNA: 3'- cgGCGGa----CUACGCGG-------CGC--------------UACUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 11793 | 0.69 | 0.339492 |
Target: 5'- uUCGC----UGCGCUGCGuUGAUGGCg -3' miRNA: 3'- cGGCGgacuACGCGGCGCuACUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 15627 | 0.69 | 0.331325 |
Target: 5'- gGCgGCCUGuuacGUGUGCCGguuauugcUGAUG-UGGCg -3' miRNA: 3'- -CGgCGGAC----UACGCGGC--------GCUACuACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 35501 | 0.71 | 0.244618 |
Target: 5'- aGCCG-CUGccGCGCCGCGcaaGAgcUGGCu -3' miRNA: 3'- -CGGCgGACuaCGCGGCGCua-CU--ACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 18751 | 0.72 | 0.214445 |
Target: 5'- aCUGcCCUGAUGauuGCCGUGgcGAUGGUa -3' miRNA: 3'- cGGC-GGACUACg--CGGCGCuaCUACCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 36106 | 0.72 | 0.203298 |
Target: 5'- aGCCauGCUUGGcaaagcUGCGCCGCGAUGGcaGCg -3' miRNA: 3'- -CGG--CGGACU------ACGCGGCGCUACUacCG- -5' |
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16913 | 3' | -57.8 | NC_004313.1 | + | 6887 | 1.12 | 0.000225 |
Target: 5'- uGCCGCCUGAUGCGCCGCGAUGAUGGCc -3' miRNA: 3'- -CGGCGGACUACGCGGCGCUACUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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