Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16913 | 5' | -56.2 | NC_004313.1 | + | 1801 | 0.66 | 0.588482 |
Target: 5'- aCGUUCCgcaguACGGCGAuccucauuuuucucaACACUGUAGAc- -3' miRNA: 3'- aGCGGGG-----UGCCGCU---------------UGUGGCAUCUug -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 8092 | 0.67 | 0.508062 |
Target: 5'- aCGCU--GCGGCGGugGCUGU-GAGCa -3' miRNA: 3'- aGCGGggUGCCGCUugUGGCAuCUUG- -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 44 | 0.68 | 0.456422 |
Target: 5'- gUCGCCCCaagccuACGGCGcGCaaggagcugGCCG-GGAACc -3' miRNA: 3'- -AGCGGGG------UGCCGCuUG---------UGGCaUCUUG- -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 27809 | 0.71 | 0.320752 |
Target: 5'- cCGUuuaaCCCACGGCGucggaguguaAAUACCGUAGGGg -3' miRNA: 3'- aGCG----GGGUGCCGC----------UUGUGGCAUCUUg -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 4795 | 0.72 | 0.248423 |
Target: 5'- cCG-CCCACGGUGAuCACCGUGGc-- -3' miRNA: 3'- aGCgGGGUGCCGCUuGUGGCAUCuug -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 4724 | 0.74 | 0.190119 |
Target: 5'- gCGCCaggaggCCACGGUGAuCACCGUGGGc- -3' miRNA: 3'- aGCGG------GGUGCCGCUuGUGGCAUCUug -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 6782 | 0.75 | 0.180005 |
Target: 5'- cUGUUCUACGGUGuuCGCCGUGGGGCg -3' miRNA: 3'- aGCGGGGUGCCGCuuGUGGCAUCUUG- -5' |
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16913 | 5' | -56.2 | NC_004313.1 | + | 6849 | 1.11 | 0.000433 |
Target: 5'- uUCGCCCCACGGCGAACACCGUAGAACa -3' miRNA: 3'- -AGCGGGGUGCCGCUUGUGGCAUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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