Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16914 | 5' | -58.1 | NC_004313.1 | + | 3770 | 0.66 | 0.482027 |
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Target: 5'- uCCG-GGGCAuuugcugcuucccuGCGGgaGUGGAGUGaCa -3' miRNA: 3'- uGGCuCCCGU--------------UGCCgaCGCUUCACgG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 8733 | 0.66 | 0.464738 |
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Target: 5'- cACgGuGuGGCAACGGCguauguggaaaGCGgcGUGCUg -3' miRNA: 3'- -UGgCuC-CCGUUGCCGa----------CGCuuCACGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 37888 | 0.66 | 0.44482 |
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Target: 5'- aACgGAGGGCAgaACGGCaucgGUGAcaggcgAGaGCCu -3' miRNA: 3'- -UGgCUCCCGU--UGCCGa---CGCU------UCaCGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 6738 | 0.66 | 0.442855 |
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Target: 5'- -gCGAGGGCAAcCGGCauaucaacGGUGCCu -3' miRNA: 3'- ugGCUCCCGUU-GCCGacgcu---UCACGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 12302 | 0.67 | 0.42539 |
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Target: 5'- uCCGGuGGCAggaAUGGCaacgGCGAAGcGCCu -3' miRNA: 3'- uGGCUcCCGU---UGCCGa---CGCUUCaCGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 5227 | 0.67 | 0.415867 |
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Target: 5'- uACCGAGGGUAACuauCUGUGgcGU-CCg -3' miRNA: 3'- -UGGCUCCCGUUGcc-GACGCuuCAcGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 34716 | 0.68 | 0.370272 |
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Target: 5'- uCCGGGGG-AAUGGCUGUc-AGUGgCCg -3' miRNA: 3'- uGGCUCCCgUUGCCGACGcuUCAC-GG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 16208 | 0.68 | 0.361572 |
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Target: 5'- gACCGGGgaaguGGCAcauCaGCUGCGu-GUGCCg -3' miRNA: 3'- -UGGCUC-----CCGUu--GcCGACGCuuCACGG- -5' |
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| 16914 | 5' | -58.1 | NC_004313.1 | + | 7064 | 1.12 | 0.000214 |
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Target: 5'- aACCGAGGGCAACGGCUGCGAAGUGCCg -3' miRNA: 3'- -UGGCUCCCGUUGCCGACGCUUCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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