Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16916 | 3' | -49 | NC_004313.1 | + | 5841 | 0.66 | 0.93167 |
Target: 5'- uACC-UGCAACUGCUGGcCAgAGCg-- -3' miRNA: 3'- -UGGuAUGUUGACGGCCuGUaUUGagg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 13145 | 0.66 | 0.93167 |
Target: 5'- aGCCugGUAUGACgacGCCGGGCGggaaucUGACaUCCu -3' miRNA: 3'- -UGG--UAUGUUGa--CGGCCUGU------AUUG-AGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 29978 | 0.66 | 0.91263 |
Target: 5'- cUCAUaaaGCGGgUGCCGGGCGUGgaaAC-CCg -3' miRNA: 3'- uGGUA---UGUUgACGGCCUGUAU---UGaGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 35217 | 0.67 | 0.905654 |
Target: 5'- aGCCGUGCGccuGCUGCgGGaaGCAggcgGAUgaUCCg -3' miRNA: 3'- -UGGUAUGU---UGACGgCC--UGUa---UUG--AGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 38790 | 0.67 | 0.882877 |
Target: 5'- cGCCAUuuGCAACUGCUGcuGCAUGAagaUCg -3' miRNA: 3'- -UGGUA--UGUUGACGGCc-UGUAUUg--AGg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 4592 | 0.67 | 0.874688 |
Target: 5'- aGCCAccuuguuuUGCGcuccACgUGCCGGACGUuucAGCUCa -3' miRNA: 3'- -UGGU--------AUGU----UG-ACGGCCUGUA---UUGAGg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 12945 | 0.68 | 0.866211 |
Target: 5'- cACCAUcCAGC-GCCGGguGCAUGACa-- -3' miRNA: 3'- -UGGUAuGUUGaCGGCC--UGUAUUGagg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 11403 | 0.68 | 0.852071 |
Target: 5'- uGCCcUGCAGCaguccguccugcugGCCGGACGgcuUUCCg -3' miRNA: 3'- -UGGuAUGUUGa-------------CGGCCUGUauuGAGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 2554 | 0.68 | 0.829607 |
Target: 5'- gGCCAUGCGACUGaCGG-CGguuuuUUCCu -3' miRNA: 3'- -UGGUAUGUUGACgGCCuGUauu--GAGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 10605 | 0.68 | 0.829607 |
Target: 5'- cCCAUcgcgGCAAUUucGCCGGAUGccACUCCg -3' miRNA: 3'- uGGUA----UGUUGA--CGGCCUGUauUGAGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 15012 | 0.69 | 0.819834 |
Target: 5'- aGCCGUAUGGCUGUgGGGuccuguCAUcccGCUCCg -3' miRNA: 3'- -UGGUAUGUUGACGgCCU------GUAu--UGAGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 10741 | 0.69 | 0.789208 |
Target: 5'- gGCCAUGCugaAGgUGCUGGACuc-GCUCg -3' miRNA: 3'- -UGGUAUG---UUgACGGCCUGuauUGAGg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 21845 | 0.71 | 0.700314 |
Target: 5'- uCCAUAUAAC-GCCGGAUAUGuucugGCUUUa -3' miRNA: 3'- uGGUAUGUUGaCGGCCUGUAU-----UGAGG- -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 14372 | 0.71 | 0.688714 |
Target: 5'- uGCCA---GACUGCCGGACugaAACUUa -3' miRNA: 3'- -UGGUaugUUGACGGCCUGua-UUGAGg -5' |
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16916 | 3' | -49 | NC_004313.1 | + | 8508 | 1.14 | 0.0014 |
Target: 5'- gACCAUACAACUGCCGGACAUAACUCCa -3' miRNA: 3'- -UGGUAUGUUGACGGCCUGUAUUGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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