Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16918 | 3' | -52.3 | NC_004313.1 | + | 27047 | 0.66 | 0.787833 |
Target: 5'- gCUGCAguacagcacUCcGA-CGCCGCGcGGUAAaGCg -3' miRNA: 3'- -GACGU---------AGuUUaGCGGCGC-CCGUUaCG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 33614 | 0.66 | 0.777397 |
Target: 5'- -gGUAUCGAAguUCGCCGCgacguucauGGGCGcuauUGUc -3' miRNA: 3'- gaCGUAGUUU--AGCGGCG---------CCCGUu---ACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 3048 | 0.67 | 0.73412 |
Target: 5'- gCUGCcgCcagcgauAUUGCCucGCGGGCAuagGCg -3' miRNA: 3'- -GACGuaGuu-----UAGCGG--CGCCCGUua-CG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 10260 | 0.67 | 0.722985 |
Target: 5'- uUGCAg-AGAUCgugGCgGCGGGCGGgcagGCa -3' miRNA: 3'- gACGUagUUUAG---CGgCGCCCGUUa---CG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 13670 | 0.67 | 0.722985 |
Target: 5'- cCUGCAUCAAcaacGUCcacaCCGaauggcCGGGCAaGUGCg -3' miRNA: 3'- -GACGUAGUU----UAGc---GGC------GCCCGU-UACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 22790 | 0.67 | 0.711749 |
Target: 5'- aCUGCAUCAGcgCaaauaGCCGCucgcgcuuccGGGCuuUGUc -3' miRNA: 3'- -GACGUAGUUuaG-----CGGCG----------CCCGuuACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 10964 | 0.67 | 0.700427 |
Target: 5'- uCUGCAUCGAGcCGCCauauuccUGGGCAucaGCc -3' miRNA: 3'- -GACGUAGUUUaGCGGc------GCCCGUua-CG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 21430 | 0.67 | 0.700427 |
Target: 5'- -gGCG-CGAAagGCUGCGGGgGAUGa -3' miRNA: 3'- gaCGUaGUUUagCGGCGCCCgUUACg -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 17340 | 0.68 | 0.6314 |
Target: 5'- gCUGguUCaAAAUCGCCaCGGuuGUAAUGCc -3' miRNA: 3'- -GACguAG-UUUAGCGGcGCC--CGUUACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 5494 | 0.68 | 0.6314 |
Target: 5'- gCUGCugaAAAUCGCUGCGGcGUAAUc- -3' miRNA: 3'- -GACGuagUUUAGCGGCGCC-CGUUAcg -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 30700 | 0.69 | 0.573768 |
Target: 5'- -cGCAUCAAugaCGCUGUGGcGCu-UGCg -3' miRNA: 3'- gaCGUAGUUua-GCGGCGCC-CGuuACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 32900 | 0.71 | 0.474014 |
Target: 5'- aCUG-GUCGAAuUUGCCGCGGGUAagGUu -3' miRNA: 3'- -GACgUAGUUU-AGCGGCGCCCGUuaCG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 25990 | 0.73 | 0.365254 |
Target: 5'- uCUGCAUUAAAUccccggCGCCGCGGGgAuuuauugaaGUGUa -3' miRNA: 3'- -GACGUAGUUUA------GCGGCGCCCgU---------UACG- -5' |
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16918 | 3' | -52.3 | NC_004313.1 | + | 10358 | 1.12 | 0.000765 |
Target: 5'- aCUGCAUCAAAUCGCCGCGGGCAAUGCc -3' miRNA: 3'- -GACGUAGUUUAGCGGCGCCCGUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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