Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16919 | 3' | -57.3 | NC_004313.1 | + | 15745 | 0.66 | 0.522507 |
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Target: 5'- aGGCGUaacgccguaCCAUCGUcaguauuaccGGCAGUUccCGCCa -3' miRNA: 3'- aCCGCG---------GGUAGCG----------CCGUUAAa-GCGGc -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 33686 | 0.67 | 0.440184 |
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Target: 5'- -aGCGCCCAugaacgUCGCGGCGAacUUCGauaCCa -3' miRNA: 3'- acCGCGGGU------AGCGCCGUUa-AAGC---GGc -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 2960 | 0.68 | 0.411252 |
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Target: 5'- gGGCugaauCCCAUCGCcuaugcccgcgaGGCAA-UUCGCUGg -3' miRNA: 3'- aCCGc----GGGUAGCG------------CCGUUaAAGCGGC- -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 20649 | 0.68 | 0.392632 |
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Target: 5'- -uGCGCCCAUUGCucaGGUGAUaacuuuggucUUCGCCc -3' miRNA: 3'- acCGCGGGUAGCG---CCGUUA----------AAGCGGc -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 12550 | 0.68 | 0.38353 |
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Target: 5'- ---aGCCgCAUUGCGGCAGacaCGCCGg -3' miRNA: 3'- accgCGG-GUAGCGCCGUUaaaGCGGC- -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 11210 | 0.69 | 0.340166 |
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Target: 5'- cGGCGaaagucgCCAUUGCGGCGcuggUgGCCGg -3' miRNA: 3'- aCCGCg------GGUAGCGCCGUuaa-AgCGGC- -5' |
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| 16919 | 3' | -57.3 | NC_004313.1 | + | 10611 | 1.09 | 0.000369 |
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Target: 5'- gUGGCGCCCAUCGCGGCAAUUUCGCCGg -3' miRNA: 3'- -ACCGCGGGUAGCGCCGUUAAAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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