Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16919 | 5' | -58.8 | NC_004313.1 | + | 12321 | 0.66 | 0.419808 |
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Target: 5'- -aCGGCGaaGCGCCuggaaacGUCAGGCGGGCUu- -3' miRNA: 3'- agGCCGU--UGUGG-------CGGUUCGCCUGGuu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 3830 | 0.66 | 0.411252 |
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Target: 5'- cUCUGGCAGCACCGCa---UGGAUgAGc -3' miRNA: 3'- -AGGCCGUUGUGGCGguucGCCUGgUU- -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 7761 | 0.66 | 0.401874 |
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Target: 5'- cUCGGCGGCaaauACCGCCcgggacAGCGGGgCAu -3' miRNA: 3'- aGGCCGUUG----UGGCGGu-----UCGCCUgGUu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 12506 | 0.66 | 0.38353 |
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Target: 5'- aUCGGCAACAUCGCCcAGCauGGAg--- -3' miRNA: 3'- aGGCCGUUGUGGCGGuUCG--CCUgguu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 3001 | 0.68 | 0.30811 |
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Target: 5'- -gCGGCAGCgACCGaaGAGCacgGGGCCAGa -3' miRNA: 3'- agGCCGUUG-UGGCggUUCG---CCUGGUU- -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 2393 | 0.69 | 0.277701 |
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Target: 5'- cCCGGCAACAacaaaacCCGCCGGG-GGAUa-- -3' miRNA: 3'- aGGCCGUUGU-------GGCGGUUCgCCUGguu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 36880 | 0.69 | 0.244556 |
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Target: 5'- aCCGGggaaAACGCCGCCAccgcGCGGcaACCAu -3' miRNA: 3'- aGGCCg---UUGUGGCGGUu---CGCC--UGGUu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 23589 | 0.7 | 0.225928 |
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Target: 5'- uUCCGGCAcUACCGgCGcuGGCGGugCu- -3' miRNA: 3'- -AGGCCGUuGUGGCgGU--UCGCCugGuu -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 13206 | 0.74 | 0.119798 |
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Target: 5'- gCCGGCGucgucauaccagGCugCGCCAGauaccaguGCGGGCCGAa -3' miRNA: 3'- aGGCCGU------------UGugGCGGUU--------CGCCUGGUU- -5' |
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| 16919 | 5' | -58.8 | NC_004313.1 | + | 10577 | 1.06 | 0.000429 |
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Target: 5'- cUCCGGCAACACCGCCAAGCGGACCAAu -3' miRNA: 3'- -AGGCCGUUGUGGCGGUUCGCCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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