Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16920 | 3' | -58.6 | NC_004313.1 | + | 14208 | 0.66 | 0.407739 |
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Target: 5'- cUGACcGaaauCCGGGugGGGCcUGCgGAUGc -3' miRNA: 3'- -ACUGuCg---GGCCCugUCCG-ACGgUUAC- -5' |
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| 16920 | 3' | -58.6 | NC_004313.1 | + | 34708 | 0.67 | 0.371214 |
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Target: 5'- cUGGCuacUCCGGGGgAauGGCUGUCAGUGg -3' miRNA: 3'- -ACUGuc-GGGCCCUgU--CCGACGGUUAC- -5' |
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| 16920 | 3' | -58.6 | NC_004313.1 | + | 15363 | 0.69 | 0.248423 |
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Target: 5'- -uACAGCCgGGuACAGGCUGCUg--- -3' miRNA: 3'- acUGUCGGgCCcUGUCCGACGGuuac -5' |
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| 16920 | 3' | -58.6 | NC_004313.1 | + | 11156 | 0.7 | 0.235684 |
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Target: 5'- uUGGCAGCCUGuccCGGGCUGUCAAa- -3' miRNA: 3'- -ACUGUCGGGCccuGUCCGACGGUUac -5' |
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| 16920 | 3' | -58.6 | NC_004313.1 | + | 38372 | 0.75 | 0.108604 |
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Target: 5'- cUGcCAGUCUGGGGCGGGCUGCa---- -3' miRNA: 3'- -ACuGUCGGGCCCUGUCCGACGguuac -5' |
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| 16920 | 3' | -58.6 | NC_004313.1 | + | 11219 | 0.97 | 0.002296 |
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Target: 5'- uUGACAGCCC-GGACAGGCUGCCAAUGg -3' miRNA: 3'- -ACUGUCGGGcCCUGUCCGACGGUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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