miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16921 5' -52.6 NC_004313.1 + 6604 0.66 0.750702
Target:  5'- uGGCCGcucgaAGCCAGGCgaaAACCCCU-CGu -3'
miRNA:   3'- gUCGGUa----UUGGUCUGg--UUGGGGAaGU- -5'
16921 5' -52.6 NC_004313.1 + 10106 0.66 0.750702
Target:  5'- -cGCCA--GCCcGGCCAuuucuuucugaaAUCCCUUCAg -3'
miRNA:   3'- guCGGUauUGGuCUGGU------------UGGGGAAGU- -5'
16921 5' -52.6 NC_004313.1 + 13807 0.66 0.739718
Target:  5'- cCGGCCugccgagAACCAG-CCGuCCCCgUUCGu -3'
miRNA:   3'- -GUCGGua-----UUGGUCuGGUuGGGG-AAGU- -5'
16921 5' -52.6 NC_004313.1 + 11834 0.68 0.637033
Target:  5'- uCAGCCAUGAUguGgguuACUcACCCUUUCGu -3'
miRNA:   3'- -GUCGGUAUUGguC----UGGuUGGGGAAGU- -5'
16921 5' -52.6 NC_004313.1 + 2200 0.68 0.625434
Target:  5'- -cGCCAUAAUCAGugCAACUgcaCCaUCGa -3'
miRNA:   3'- guCGGUAUUGGUCugGUUGG---GGaAGU- -5'
16921 5' -52.6 NC_004313.1 + 2421 0.71 0.437033
Target:  5'- cCAGCCAcGACCAGAacaugcaCAgcGCCCCggCAa -3'
miRNA:   3'- -GUCGGUaUUGGUCUg------GU--UGGGGaaGU- -5'
16921 5' -52.6 NC_004313.1 + 11417 1.09 0.001082
Target:  5'- cCAGCCAUAACCAGACCAACCCCUUCAa -3'
miRNA:   3'- -GUCGGUAUUGGUCUGGUUGGGGAAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.