Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16924 | 5' | -57.5 | NC_004313.1 | + | 24246 | 0.66 | 0.441527 |
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Target: 5'- aGCGCCA--ACGguuaCGUCAGCGCAUa -3' miRNA: 3'- gCGCGGUagUGCguc-GUAGUCGCGUA- -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 6829 | 0.66 | 0.43159 |
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Target: 5'- uCGCgGCgCAUCAgGCGGCAgCGGCugGCGUg -3' miRNA: 3'- -GCG-CG-GUAGUgCGUCGUaGUCG--CGUA- -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 22772 | 0.67 | 0.393195 |
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Target: 5'- aGCGCgGUCGuaacaGauacuGCAUCAGCGCAa -3' miRNA: 3'- gCGCGgUAGUg----Cgu---CGUAGUCGCGUa -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 5203 | 0.68 | 0.348432 |
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Target: 5'- uGCGCC-UUACGCAGCGuguaaaUCAGCuuaaugaugGCAUc -3' miRNA: 3'- gCGCGGuAGUGCGUCGU------AGUCG---------CGUA- -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 11731 | 0.7 | 0.243123 |
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Target: 5'- -cCGCCAUCaACGCAGCG-CAGCGa-- -3' miRNA: 3'- gcGCGGUAG-UGCGUCGUaGUCGCgua -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 7710 | 0.71 | 0.230445 |
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Target: 5'- uGCGCCGgacaUCAC-CAGCGgccucgCGGCGCAg -3' miRNA: 3'- gCGCGGU----AGUGcGUCGUa-----GUCGCGUa -5' |
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| 16924 | 5' | -57.5 | NC_004313.1 | + | 16198 | 1.05 | 0.000595 |
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Target: 5'- gCGCGCCAUCACGCAGCAUCAGCGCAUu -3' miRNA: 3'- -GCGCGGUAGUGCGUCGUAGUCGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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