Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16925 | 3' | -48.8 | NC_004313.1 | + | 24376 | 0.65 | 0.953148 |
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Target: 5'- -gCGCUGUAUAGUcauuucagCACCGCAa -3' miRNA: 3'- ggGUGGCAUGUCAaauua---GUGGCGUc -5' |
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| 16925 | 3' | -48.8 | NC_004313.1 | + | 1098 | 0.67 | 0.92773 |
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Target: 5'- aCC-CCGgggACGGggc-AUCACCGCAc -3' miRNA: 3'- gGGuGGCa--UGUCaaauUAGUGGCGUc -5' |
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| 16925 | 3' | -48.8 | NC_004313.1 | + | 6772 | 0.67 | 0.901078 |
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Target: 5'- cCCCGCCuuucuguucUACGGUgu--UCGCCGUGGg -3' miRNA: 3'- -GGGUGGc--------AUGUCAaauuAGUGGCGUC- -5' |
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| 16925 | 3' | -48.8 | NC_004313.1 | + | 4796 | 0.69 | 0.833646 |
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Target: 5'- aCCGCCc-ACGGU--GAUCACCGUGGc -3' miRNA: 3'- gGGUGGcaUGUCAaaUUAGUGGCGUC- -5' |
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| 16925 | 3' | -48.8 | NC_004313.1 | + | 8147 | 0.71 | 0.717496 |
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Target: 5'- gCCACCGccGCAGcgUcAUCACCGCuGg -3' miRNA: 3'- gGGUGGCa-UGUCaaAuUAGUGGCGuC- -5' |
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| 16925 | 3' | -48.8 | NC_004313.1 | + | 17450 | 1.12 | 0.002397 |
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Target: 5'- cCCCACCGUACAGUUUAAUCACCGCAGu -3' miRNA: 3'- -GGGUGGCAUGUCAAAUUAGUGGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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