Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16926 | 3' | -47 | NC_004313.1 | + | 23490 | 0.67 | 0.946025 |
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Target: 5'- gGCaCCAugaagcaGGGCAGCG-CGACAAGCGu -3' miRNA: 3'- -CG-GGUua-----CUCGUUGUaGCUGUUUGUc -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 9659 | 0.69 | 0.909361 |
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Target: 5'- uUCCGGUGAcgGCGGCGUCcGGCuGGACAGu -3' miRNA: 3'- cGGGUUACU--CGUUGUAG-CUG-UUUGUC- -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 10589 | 0.69 | 0.893765 |
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Target: 5'- cGCCgGAUGccacuccGGCAACAcCGcCAAGCGGa -3' miRNA: 3'- -CGGgUUAC-------UCGUUGUaGCuGUUUGUC- -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 25028 | 0.72 | 0.748373 |
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Target: 5'- aGCUCAAUGAGCAACcgaauUCG-CAAugcuCAGg -3' miRNA: 3'- -CGGGUUACUCGUUGu----AGCuGUUu---GUC- -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 12591 | 0.72 | 0.736994 |
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Target: 5'- cGCCCGGUG-GCAuCAgaaUGACAGACAa -3' miRNA: 3'- -CGGGUUACuCGUuGUa--GCUGUUUGUc -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 12495 | 0.73 | 0.702163 |
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Target: 5'- cGCCCAgcAUGgaGGCuGCAUCGGCAAugAc -3' miRNA: 3'- -CGGGU--UAC--UCGuUGUAGCUGUUugUc -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 12192 | 0.76 | 0.547511 |
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Target: 5'- gGCgaAAUGcagGGCAGCAUUGACGGACAGg -3' miRNA: 3'- -CGggUUAC---UCGUUGUAGCUGUUUGUC- -5' |
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| 16926 | 3' | -47 | NC_004313.1 | + | 24516 | 1.13 | 0.00256 |
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Target: 5'- uGCCCAAUGAGCAACAUCGACAAACAGg -3' miRNA: 3'- -CGGGUUACUCGUUGUAGCUGUUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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