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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16927 | 5' | -46.2 | NC_004313.1 | + | 18724 | 0.66 | 0.978679 |
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Target: 5'- uGUCUGuuGCGGAAAUAGA-CGCUGc- -3' miRNA: 3'- -CGGACuuUGUUUUUGUCUaGCGGCuu -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 10686 | 0.67 | 0.972732 |
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Target: 5'- uCCUGAAGCuGAAucccCGGAaacUCGCUGAGg -3' miRNA: 3'- cGGACUUUGuUUUu---GUCU---AGCGGCUU- -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 29376 | 0.67 | 0.965671 |
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Target: 5'- cGCCUgcugGAAGCGAuuGAGCAGAUCaCCa-- -3' miRNA: 3'- -CGGA----CUUUGUU--UUUGUCUAGcGGcuu -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 14155 | 0.68 | 0.93706 |
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Target: 5'- --aUGAAACGcu-GguGAUCGCCGAAg -3' miRNA: 3'- cggACUUUGUuuuUguCUAGCGGCUU- -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 5573 | 0.71 | 0.84441 |
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Target: 5'- uCCUGAAACAAGAucuCAaauGGUCGCCa-- -3' miRNA: 3'- cGGACUUUGUUUUu--GU---CUAGCGGcuu -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 33543 | 0.73 | 0.760325 |
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Target: 5'- aUUUGAAACAGGGGCGGGUgcaGCCGAc -3' miRNA: 3'- cGGACUUUGUUUUUGUCUAg--CGGCUu -5' |
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| 16927 | 5' | -46.2 | NC_004313.1 | + | 26434 | 1.1 | 0.004857 |
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Target: 5'- cGCCUGAAACAAAAACAGAUCGCCGAAu -3' miRNA: 3'- -CGGACUUUGUUUUUGUCUAGCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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