Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16929 | 3' | -47.2 | NC_004313.1 | + | 20603 | 0.66 | 0.972193 |
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Target: 5'- uGGGCGc--AGGCcGGGCGUCUgaUUGGg -3' miRNA: 3'- gUCCGCcaaUUUGuCUCGUAGA--AACC- -5' |
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| 16929 | 3' | -47.2 | NC_004313.1 | + | 3055 | 0.66 | 0.968814 |
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Target: 5'- uCGGGUGuGUUGcguACAGAGCAgacgCUgucGGa -3' miRNA: 3'- -GUCCGC-CAAUu--UGUCUCGUa---GAaa-CC- -5' |
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| 16929 | 3' | -47.2 | NC_004313.1 | + | 10277 | 0.68 | 0.924799 |
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Target: 5'- gCGGGCGGgcAGGCAG-GCAUUg---- -3' miRNA: 3'- -GUCCGCCaaUUUGUCuCGUAGaaacc -5' |
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| 16929 | 3' | -47.2 | NC_004313.1 | + | 18175 | 0.68 | 0.911485 |
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Target: 5'- gCAGGCGGggcUAucuaucagaauAACGGcGaCAUCUUUGGu -3' miRNA: 3'- -GUCCGCCa--AU-----------UUGUCuC-GUAGAAACC- -5' |
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| 16929 | 3' | -47.2 | NC_004313.1 | + | 24177 | 0.7 | 0.863938 |
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Target: 5'- gGGaGCGGgcAGACAGAGCAgaaaagCgcgUGGc -3' miRNA: 3'- gUC-CGCCaaUUUGUCUCGUa-----Gaa-ACC- -5' |
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| 16929 | 3' | -47.2 | NC_004313.1 | + | 29256 | 1.11 | 0.00328 |
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Target: 5'- gCAGGCGGUUAAACAGAGCAUCUUUGGu -3' miRNA: 3'- -GUCCGCCAAUUUGUCUCGUAGAAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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