Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16931 | 3' | -61.7 | NC_004313.1 | + | 93 | 0.67 | 0.289894 |
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Target: 5'- uUGGGg--CgACGCCCGGaacgcCCCGCc -3' miRNA: 3'- gGCCCaaaGgUGCGGGCCgu---GGGCGa -5' |
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| 16931 | 3' | -61.7 | NC_004313.1 | + | 5365 | 0.68 | 0.256225 |
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Target: 5'- aCgGGGUUacaCCAuCGUugaccguaUCGGCACCCGCa -3' miRNA: 3'- -GgCCCAAa--GGU-GCG--------GGCCGUGGGCGa -5' |
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| 16931 | 3' | -61.7 | NC_004313.1 | + | 6300 | 0.71 | 0.154976 |
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Target: 5'- aCCGGGUUgcgcuuuuuuggcCCACGUCCGG-AUCUGCUc -3' miRNA: 3'- -GGCCCAAa------------GGUGCGGGCCgUGGGCGA- -5' |
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| 16931 | 3' | -61.7 | NC_004313.1 | + | 4492 | 0.77 | 0.050255 |
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Target: 5'- -aGGGUUUCCACcuGCCCGGCAagcuugcccuuuuCCUGCUc -3' miRNA: 3'- ggCCCAAAGGUG--CGGGCCGU-------------GGGCGA- -5' |
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| 16931 | 3' | -61.7 | NC_004313.1 | + | 37381 | 0.79 | 0.040151 |
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Target: 5'- gCCGGGcUUUCUGCGCgCUGGUAUCCGCa -3' miRNA: 3'- -GGCCC-AAAGGUGCG-GGCCGUGGGCGa -5' |
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| 16931 | 3' | -61.7 | NC_004313.1 | + | 30051 | 1.08 | 0.000225 |
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Target: 5'- uCCGGGUUUCCACGCCCGGCACCCGCUu -3' miRNA: 3'- -GGCCCAAAGGUGCGGGCCGUGGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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