Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16934 | 5' | -51.6 | NC_004313.1 | + | 9794 | 0.66 | 0.820476 |
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Target: 5'- uGCCAGuuGCGCUGccGAGGagcGugGAa -3' miRNA: 3'- -UGGUUguCGCGAUuaCUCCa--CugCUg -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 11220 | 0.67 | 0.769057 |
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Target: 5'- cGCCAuuGCGGCGCU-----GGUGGcCGGCg -3' miRNA: 3'- -UGGU--UGUCGCGAuuacuCCACU-GCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 16126 | 0.67 | 0.769057 |
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Target: 5'- aGCUAAauGCGCUGAUGcuGcGUGAUGGCg -3' miRNA: 3'- -UGGUUguCGCGAUUACu-C-CACUGCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 6167 | 0.67 | 0.7362 |
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Target: 5'- uCCGGCGGCGUgucGAUGuuccGGcUGAUGACu -3' miRNA: 3'- uGGUUGUCGCGa--UUACu---CC-ACUGCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 8291 | 0.68 | 0.706854 |
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Target: 5'- uGCCAGCGGCGUgaagGGGGcUGgugcgccagcucuuaACGACg -3' miRNA: 3'- -UGGUUGUCGCGauuaCUCC-AC---------------UGCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 36972 | 0.68 | 0.666515 |
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Target: 5'- cGCCaAACAGCaGCcuGUGAaugguugccgcgcGGUGGCGGCg -3' miRNA: 3'- -UGG-UUGUCG-CGauUACU-------------CCACUGCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 1199 | 0.74 | 0.373672 |
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Target: 5'- cGCCGACAGcCGCUGAUGuGGgcaauaaccACGGCg -3' miRNA: 3'- -UGGUUGUC-GCGAUUACuCCac-------UGCUG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 24639 | 0.8 | 0.142884 |
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Target: 5'- cCCAGCAGUuguGCUgGAUGAGGUGACGuACa -3' miRNA: 3'- uGGUUGUCG---CGA-UUACUCCACUGC-UG- -5' |
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| 16934 | 5' | -51.6 | NC_004313.1 | + | 33322 | 1.09 | 0.00141 |
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Target: 5'- gACCAACAGCGCUAAUGAGGUGACGACc -3' miRNA: 3'- -UGGUUGUCGCGAUUACUCCACUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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