Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16936 | 3' | -49.2 | NC_004313.1 | + | 12114 | 0.66 | 0.895714 |
Target: 5'- ----gAUUACGcaGACAgGCGGGAUCGUCu -3' miRNA: 3'- uugugUAGUGC--UUGU-CGUCCUAGUAG- -5' |
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16936 | 3' | -49.2 | NC_004313.1 | + | 8994 | 0.67 | 0.844246 |
Target: 5'- cAACAUAUCGCcaGAugGaGCGGGAaggCAUCg -3' miRNA: 3'- -UUGUGUAGUG--CUugU-CGUCCUa--GUAG- -5' |
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16936 | 3' | -49.2 | NC_004313.1 | + | 12203 | 0.68 | 0.824875 |
Target: 5'- gGGCAgCAUUgACGGACAGguCAGGAUCAcUCa -3' miRNA: 3'- -UUGU-GUAG-UGCUUGUC--GUCCUAGU-AG- -5' |
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16936 | 3' | -49.2 | NC_004313.1 | + | 11 | 0.68 | 0.804545 |
Target: 5'- cGCAUAUggCugGAACGGCGGGG-CGUUc -3' miRNA: 3'- uUGUGUA--GugCUUGUCGUCCUaGUAG- -5' |
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16936 | 3' | -49.2 | NC_004313.1 | + | 26321 | 0.74 | 0.46353 |
Target: 5'- -cCGCAUUACGAuccaacaggcaaccCAGCAGGAUCAUg -3' miRNA: 3'- uuGUGUAGUGCUu-------------GUCGUCCUAGUAg -5' |
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16936 | 3' | -49.2 | NC_004313.1 | + | 34696 | 1.07 | 0.003043 |
Target: 5'- gAACACAUCACGAACAGCAGGAUCAUCc -3' miRNA: 3'- -UUGUGUAGUGCUUGUCGUCCUAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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