Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16938 | 5' | -46 | NC_004313.1 | + | 10254 | 0.66 | 0.987119 |
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Target: 5'- aGGGAAuugcAGAGAucguggcggcGGGCgGgCAGGCAGg -3' miRNA: 3'- aCCUUUu---UCUUU----------UCCGgCgGUUUGUC- -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 7255 | 0.67 | 0.97499 |
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Target: 5'- aGGAAAucuGGAAGGcGCUGCUggGguGg -3' miRNA: 3'- aCCUUUuu-CUUUUC-CGGCGGuuUguC- -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 17769 | 0.67 | 0.97499 |
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Target: 5'- aUGGAGGGAGGcuAAAcGGuCCGUUAGGCAu -3' miRNA: 3'- -ACCUUUUUCU--UUU-CC-GGCGGUUUGUc -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 27456 | 0.67 | 0.97499 |
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Target: 5'- cGGuaucuGGcGAAGGUugcaCGCCAGACGGg -3' miRNA: 3'- aCCuuuu-UCuUUUCCG----GCGGUUUGUC- -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 5561 | 0.7 | 0.904826 |
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Target: 5'- aGGGAAucaugauccugaaacAAGAucucaaauGGUCGCCAGACGGu -3' miRNA: 3'- aCCUUU---------------UUCUuuu-----CCGGCGGUUUGUC- -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 21439 | 0.7 | 0.901838 |
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Target: 5'- gUGGAGAAAGGcgcgAAAGGCUGCgGGGgAu -3' miRNA: 3'- -ACCUUUUUCU----UUUCCGGCGgUUUgUc -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 4417 | 0.73 | 0.756621 |
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Target: 5'- aGGAAAAGGgcAAGcUUGCCGGGCAGg -3' miRNA: 3'- aCCUUUUUCuuUUCcGGCGGUUUGUC- -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 19109 | 0.74 | 0.721857 |
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Target: 5'- aGGAAAAAGAAAAGGCggguaauaaaccCGCCuGAAUAu -3' miRNA: 3'- aCCUUUUUCUUUUCCG------------GCGG-UUUGUc -5' |
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| 16938 | 5' | -46 | NC_004313.1 | + | 36989 | 1.1 | 0.005259 |
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Target: 5'- gUGGAAAAAGAAAAGGCCGCCAAACAGc -3' miRNA: 3'- -ACCUUUUUCUUUUCCGGCGGUUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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