miRNA: 3'- aGACUA-CACa--UUCGCgUG-UGGUGAUG- -5'
miRNA: 3'- -AGACUACACA-UUCGCGugU-GGUGAUg -5'
miRNA: 3'- aGACUACaCAuUCgCGUGUGGUGAUG- -5'
miRNA: 3'- -AGACUACACAUuCGCGUGUGGUGAUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16939 | 3' | -49.7 | NC_004313.1 | + | 1799 | 0.66 | 0.896731 |
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Target: 5'- aCUGAUgGUGcggAGGUGgAUuACCGCUACa -3' miRNA: 3'- aGACUA-CACa--UUCGCgUG-UGGUGAUG- -5' |
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| 16939 | 3' | -49.7 | NC_004313.1 | + | 38891 | 0.7 | 0.708015 |
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Target: 5'- uUCUGGUGUGUgGGGUGUugAaCUACUGg -3' miRNA: 3'- -AGACUACACA-UUCGCGugU-GGUGAUg -5' |
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| 16939 | 3' | -49.7 | NC_004313.1 | + | 16640 | 0.7 | 0.661155 |
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Target: 5'- cUUGAUGcGUuGGgGUAUACCGCUGCc -3' miRNA: 3'- aGACUACaCAuUCgCGUGUGGUGAUG- -5' |
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| 16939 | 3' | -49.7 | NC_004313.1 | + | 988 | 1.02 | 0.007313 |
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Target: 5'- gUCUGAUGUGUA-GCGCACACCACUACu -3' miRNA: 3'- -AGACUACACAUuCGCGUGUGGUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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