Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16940 | 3' | -59.4 | NC_004313.1 | + | 37865 | 0.66 | 0.390353 |
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Target: 5'- aACCACGCU-GCGGAaCgGGCGGcAUGa -3' miRNA: 3'- cUGGUGCGGuCGUCUcGaCCGCC-UAC- -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 15995 | 0.67 | 0.330063 |
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Target: 5'- uGACCAuauuuugcCGCUGGCAccuguuGCUGGCGGcgGa -3' miRNA: 3'- -CUGGU--------GCGGUCGUcu----CGACCGCCuaC- -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 15072 | 0.67 | 0.314147 |
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Target: 5'- -cCCGCGaauacaugCAGCAGGcGCUGGCguGGAUGa -3' miRNA: 3'- cuGGUGCg-------GUCGUCU-CGACCG--CCUAC- -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 34595 | 0.68 | 0.284075 |
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Target: 5'- uGCgGCGCgguccCGGCGGuaucacagccGCUGGCGGAUGa -3' miRNA: 3'- cUGgUGCG-----GUCGUCu---------CGACCGCCUAC- -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 38346 | 0.68 | 0.276922 |
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Target: 5'- -uCCACGCgGGCGGcGgaGGUGGAUu -3' miRNA: 3'- cuGGUGCGgUCGUCuCgaCCGCCUAc -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 13352 | 0.69 | 0.230836 |
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Target: 5'- cGAUCAC-CCGGCAGuGGcCUGGUGGAg- -3' miRNA: 3'- -CUGGUGcGGUCGUC-UC-GACCGCCUac -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 35217 | 0.7 | 0.19664 |
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Target: 5'- aGCCguGCGCCugcuGCGGgaAGCaGGCGGAUGa -3' miRNA: 3'- cUGG--UGCGGu---CGUC--UCGaCCGCCUAC- -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 35507 | 0.77 | 0.0652 |
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Target: 5'- uGCCGCGCCGcGCaAGAGCUGGCuGGGa- -3' miRNA: 3'- cUGGUGCGGU-CG-UCUCGACCG-CCUac -5' |
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| 16940 | 3' | -59.4 | NC_004313.1 | + | 2475 | 1.07 | 0.000321 |
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Target: 5'- uGACCACGCCAGCAGAGCUGGCGGAUGc -3' miRNA: 3'- -CUGGUGCGGUCGUCUCGACCGCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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