Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16943 | 5' | -50 | NC_004313.1 | + | 2554 | 0.66 | 0.876657 |
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Target: 5'- gGCCAuGCGAcUGAcggcgguuuuuuccUGCGUCAGagUGCUGg -3' miRNA: 3'- -CGGU-CGUU-ACU--------------ACGCAGUUa-ACGGCa -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 16571 | 0.66 | 0.870787 |
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Target: 5'- cGCCAGCAAcggcugGAUGCaaaggCAaauGUUGCUGg -3' miRNA: 3'- -CGGUCGUUa-----CUACGca---GU---UAACGGCa -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 4954 | 0.66 | 0.86216 |
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Target: 5'- uCCAGCAuuuucuggGUGGccUGCGg--GUUGCCGUa -3' miRNA: 3'- cGGUCGU--------UACU--ACGCaguUAACGGCA- -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 10621 | 0.67 | 0.844087 |
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Target: 5'- cCCGGCAAUGGUggcgcccaucGCGgCAAUUucGCCGg -3' miRNA: 3'- cGGUCGUUACUA----------CGCaGUUAA--CGGCa -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 1167 | 0.68 | 0.784095 |
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Target: 5'- cGCaguGCGGUGAUGCcccGUCcccggGGUUGCCGa -3' miRNA: 3'- -CGgu-CGUUACUACG---CAG-----UUAACGGCa -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 8079 | 0.69 | 0.729002 |
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Target: 5'- aCCAGCGGUGAugacgcUGCGgCGGUgGCUGUg -3' miRNA: 3'- cGGUCGUUACU------ACGCaGUUAaCGGCA- -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 35990 | 0.69 | 0.729002 |
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Target: 5'- cGUguGCGAUGGUGUGUgAAUcacuuuaauugUGCUGUa -3' miRNA: 3'- -CGguCGUUACUACGCAgUUA-----------ACGGCA- -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 10555 | 0.69 | 0.706086 |
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Target: 5'- uGCC-GCGAUGG-GCGcCAccAUUGCCGg -3' miRNA: 3'- -CGGuCGUUACUaCGCaGU--UAACGGCa -5' |
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| 16943 | 5' | -50 | NC_004313.1 | + | 7812 | 1.1 | 0.001754 |
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Target: 5'- cGCCAGCAAUGAUGCGUCAAUUGCCGUc -3' miRNA: 3'- -CGGUCGUUACUACGCAGUUAACGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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