Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16944 | 5' | -55.5 | NC_004313.1 | + | 24850 | 0.66 | 0.591748 |
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Target: 5'- cCCCAGACauacCCgcGCUGAU-AAUUGGCa -3' miRNA: 3'- -GGGUCUGc---GGa-CGACUAcUUGACCGa -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 39266 | 0.66 | 0.591748 |
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Target: 5'- aCUCAGggaACGCCUGCUGuaAUGuacuAACUGGa- -3' miRNA: 3'- -GGGUC---UGCGGACGAC--UAC----UUGACCga -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 24281 | 0.68 | 0.482514 |
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Target: 5'- aCCCGaaugugcuGGCG-CUGCUgGAUGAGCUGGaCa -3' miRNA: 3'- -GGGU--------CUGCgGACGA-CUACUUGACC-Ga -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 15139 | 0.7 | 0.348432 |
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Target: 5'- gCCAG-CGCCUGCUGcAUGuauucGCgGGCa -3' miRNA: 3'- gGGUCuGCGGACGAC-UACu----UGaCCGa -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 15420 | 0.71 | 0.331574 |
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Target: 5'- gCCCGGAauaugcUGCCUGauaugugUGAUGAggACUGGCUu -3' miRNA: 3'- -GGGUCU------GCGGACg------ACUACU--UGACCGA- -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 37413 | 0.71 | 0.323372 |
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Target: 5'- aCCAGcCGCCUGCUGA--AAUUGGg- -3' miRNA: 3'- gGGUCuGCGGACGACUacUUGACCga -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 33125 | 0.72 | 0.270213 |
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Target: 5'- aCCAGcgaaaucauuACuaCUGCUGGUGGACUGGUc -3' miRNA: 3'- gGGUC----------UGcgGACGACUACUUGACCGa -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 35213 | 0.75 | 0.17038 |
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Target: 5'- uCCCAGccguGCGCCUGCUGcgGGaaGCaGGCg -3' miRNA: 3'- -GGGUC----UGCGGACGACuaCU--UGaCCGa -5' |
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| 16944 | 5' | -55.5 | NC_004313.1 | + | 8568 | 1.1 | 0.000451 |
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Target: 5'- aCCCAGACGCCUGCUGAUGAACUGGCUg -3' miRNA: 3'- -GGGUCUGCGGACGACUACUUGACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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