Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16948 | 3' | -53 | NC_004313.1 | + | 30 | 0.66 | 0.775189 |
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Target: 5'- gGGGCGUUc-CGGGCGUcgccccaAGCcu-ACGGc -3' miRNA: 3'- -CCCGUAAcaGCCCGCG-------UCGuuuUGCC- -5' |
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| 16948 | 3' | -53 | NC_004313.1 | + | 4423 | 0.67 | 0.722706 |
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Target: 5'- aGGGCAagcUUGcCGGGCaGguGgAaacccuGAACGGg -3' miRNA: 3'- -CCCGU---AACaGCCCG-CguCgU------UUUGCC- -5' |
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| 16948 | 3' | -53 | NC_004313.1 | + | 39081 | 0.67 | 0.722706 |
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Target: 5'- uGGCuaaGUgGGGCGUuGguGGGCGGa -3' miRNA: 3'- cCCGuaaCAgCCCGCGuCguUUUGCC- -5' |
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| 16948 | 3' | -53 | NC_004313.1 | + | 8697 | 0.68 | 0.66678 |
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Target: 5'- cGGUcuggGUCGGGCGCGcCGGAucguuACGGa -3' miRNA: 3'- cCCGuaa-CAGCCCGCGUcGUUU-----UGCC- -5' |
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| 16948 | 3' | -53 | NC_004313.1 | + | 14901 | 0.7 | 0.546995 |
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Target: 5'- cGGGCAgugGUgauuucucuuuuuacCuGGCGCcgGGCGGAGCGGg -3' miRNA: 3'- -CCCGUaa-CA---------------GcCCGCG--UCGUUUUGCC- -5' |
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| 16948 | 3' | -53 | NC_004313.1 | + | 13496 | 1.13 | 0.000679 |
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Target: 5'- cGGGCAUUGUCGGGCGCAGCAAAACGGc -3' miRNA: 3'- -CCCGUAACAGCCCGCGUCGUUUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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