Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16949 | 5' | -52.3 | NC_004313.1 | + | 600 | 0.66 | 0.77449 |
Target: 5'- ------cAGGCCUGcCAGCGUcacauugaugauaugGCGGCg -3' miRNA: 3'- cuuaaacUUCGGGCaGUCGCA---------------CGCCG- -5' |
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16949 | 5' | -52.3 | NC_004313.1 | + | 9872 | 0.67 | 0.726679 |
Target: 5'- ----cUGGAGCUC--CGGCGgGCGGCg -3' miRNA: 3'- cuuaaACUUCGGGcaGUCGCaCGCCG- -5' |
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16949 | 5' | -52.3 | NC_004313.1 | + | 27462 | 0.71 | 0.467281 |
Target: 5'- ----gUGAAGuCuuGUCGGCGUcCGGCu -3' miRNA: 3'- cuuaaACUUC-GggCAGUCGCAcGCCG- -5' |
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16949 | 5' | -52.3 | NC_004313.1 | + | 7864 | 0.73 | 0.387257 |
Target: 5'- ---gUUGAuuauGCCCGUCAGauuUGCGGUg -3' miRNA: 3'- cuuaAACUu---CGGGCAGUCgc-ACGCCG- -5' |
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16949 | 5' | -52.3 | NC_004313.1 | + | 14018 | 1.13 | 0.000689 |
Target: 5'- uGAAUUUGAAGCCCGUCAGCGUGCGGCg -3' miRNA: 3'- -CUUAAACUUCGGGCAGUCGCACGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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