Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16951 | 5' | -56.6 | NC_004313.1 | + | 16068 | 0.66 | 0.55753 |
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Target: 5'- gCCAGCaacaGGUGCCAGCgGCAaaaUauGGUCAc -3' miRNA: 3'- -GGUCG----UCGCGGUCG-UGUa--AgcCCAGU- -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 11181 | 0.66 | 0.546558 |
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Target: 5'- gCCAGCAGCGCC-GCGCcgaacUUCGccgCAg -3' miRNA: 3'- -GGUCGUCGCGGuCGUGu----AAGCccaGU- -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 2482 | 0.67 | 0.472092 |
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Target: 5'- gCCAGCAGaGCUGGCGgAUgcuaUCGGGcugUCAu -3' miRNA: 3'- -GGUCGUCgCGGUCGUgUA----AGCCC---AGU- -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 34199 | 0.68 | 0.440771 |
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Target: 5'- uCCAGC-GCGCCAGCcggagcaucaaugGCAUauugUGGGcCAg -3' miRNA: 3'- -GGUCGuCGCGGUCG-------------UGUAa---GCCCaGU- -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 10159 | 0.7 | 0.321169 |
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Target: 5'- aCgGGCAGCGCCAGCGCcccaagaacaGcGGUCc -3' miRNA: 3'- -GgUCGUCGCGGUCGUGuaag------C-CCAGu -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 16564 | 0.71 | 0.2577 |
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Target: 5'- aCAGCAGCGCCAGCAaCGgcUGGaugCAa -3' miRNA: 3'- gGUCGUCGCGGUCGU-GUaaGCCca-GU- -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 34856 | 0.73 | 0.208224 |
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Target: 5'- --cGCAGUGCCAGCACAgUCGGa--- -3' miRNA: 3'- gguCGUCGCGGUCGUGUaAGCCcagu -5' |
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| 16951 | 5' | -56.6 | NC_004313.1 | + | 24218 | 1.1 | 0.00041 |
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Target: 5'- uCCAGCAGCGCCAGCACAUUCGGGUCAc -3' miRNA: 3'- -GGUCGUCGCGGUCGUGUAAGCCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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