Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16953 | 3' | -52.4 | NC_004313.1 | + | 36727 | 0.67 | 0.653354 |
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Target: 5'- uGGCUGGCgcGGUAGuuauugguuccUGCCAGUGAa-- -3' miRNA: 3'- uUCGACCGa-CUAUU-----------ACGGUCGCUagu -5' |
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| 16953 | 3' | -52.4 | NC_004313.1 | + | 18153 | 0.68 | 0.58251 |
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Target: 5'- -cGCUGGCUGuacaGAUGCCAucccUGAUCGu -3' miRNA: 3'- uuCGACCGACua--UUACGGUc---GCUAGU- -5' |
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| 16953 | 3' | -52.4 | NC_004313.1 | + | 29109 | 0.69 | 0.502112 |
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Target: 5'- -cGCUGGCUGGUG--GCU-GCGAUUAa -3' miRNA: 3'- uuCGACCGACUAUuaCGGuCGCUAGU- -5' |
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| 16953 | 3' | -52.4 | NC_004313.1 | + | 22574 | 0.7 | 0.490998 |
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Target: 5'- -cGCUGGCUGA---UGCCAG-GcAUCAg -3' miRNA: 3'- uuCGACCGACUauuACGGUCgC-UAGU- -5' |
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| 16953 | 3' | -52.4 | NC_004313.1 | + | 4745 | 0.7 | 0.48 |
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Target: 5'- cAGCaGGCUGAgAAUGCU-GCGAUCc -3' miRNA: 3'- uUCGaCCGACUaUUACGGuCGCUAGu -5' |
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| 16953 | 3' | -52.4 | NC_004313.1 | + | 31065 | 1.07 | 0.001274 |
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Target: 5'- cAAGCUGGCUGAUAAUGCCAGCGAUCAg -3' miRNA: 3'- -UUCGACCGACUAUUACGGUCGCUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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