Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16953 | 5' | -54 | NC_004313.1 | + | 25435 | 0.67 | 0.590721 |
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Target: 5'- cGCgGcGUUCAGUUGCCAGCAUGgucagaucUGCa -3' miRNA: 3'- -CGaC-CGGGUCGAUGGUCGUAUa-------ACGa -5' |
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| 16953 | 5' | -54 | NC_004313.1 | + | 11932 | 0.68 | 0.522588 |
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Target: 5'- gGCUGGCgCgGGC-GCCuGCGUGaUGCa -3' miRNA: 3'- -CGACCG-GgUCGaUGGuCGUAUaACGa -5' |
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| 16953 | 5' | -54 | NC_004313.1 | + | 13703 | 0.68 | 0.489641 |
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Target: 5'- gGCUGaGCCUcaauaacggcGGCUGCCGGCGcgccUGCa -3' miRNA: 3'- -CGAC-CGGG----------UCGAUGGUCGUaua-ACGa -5' |
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| 16953 | 5' | -54 | NC_004313.1 | + | 3070 | 0.72 | 0.298584 |
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Target: 5'- uCUGGCCCcguGCUcuucggucgcugccGCCAGCgAUAUUGCc -3' miRNA: 3'- cGACCGGGu--CGA--------------UGGUCG-UAUAACGa -5' |
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| 16953 | 5' | -54 | NC_004313.1 | + | 31099 | 1.1 | 0.000541 |
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Target: 5'- uGCUGGCCCAGCUACCAGCAUAUUGCUg -3' miRNA: 3'- -CGACCGGGUCGAUGGUCGUAUAACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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